egfp pp1cb d63a mypt1 Search Results


egfp pp1cb d63a mypt1  (Addgene inc)
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Addgene inc egfp pp1cb d63a mypt1
(A) Depiction of relevant clusters of PCDH7 isoform-c interactome determined by GFP mediated affinity pulldown followed by LC-MS/MS. Red inset depicts a set of phosphatase subunits and phosphatase regulatory proteins, green inset highlighting components of myosin complexes, and blue inset showing members of ERM proteins. (B) Immunostaining of RPE1 cells expressing PCDH7-GFP (green), <t>MYPT1</t> (PPP1R12A) (red) and fluorescent phalloidin (blue). (C) Immunoblotting for GFP pulldown of <t>EGFP-PP1CB-MYPT1</t> fusion, EGFP-PP1CB*-MYPT1 <t>(D63A)</t> mutant and control construct with cotransfection of PCDH7c-V5 construct. Membranes were blotted using anti-V5, anti-MYPT1, anti-GFP and anti-tubulin antibodies. I (Input), FT(Flow Through), E (Elution) (D) Quantification of proximity ligation assay (PLA) showing fluorescence dots per cell for PCDH7- GFP (anti-GFP) and MYPT1 (anti-MYPT1) interaction (n=29) along with single primary antibody controls, MYPT1 (n=30) and GFP (n=30). ****p<0.0001.
Egfp Pp1cb D63a Mypt1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/egfp+pp1cb+d63a+mypt1/pLEX_307+(Plasmid+%2341392)/bio_rxiv__2021__09__21__460794-205-15-11
Average 96 stars, based on 1 article reviews
egfp pp1cb d63a mypt1 - by Bioz Stars, 2026-09
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(A) Depiction of relevant clusters of PCDH7 isoform-c interactome determined by GFP mediated affinity pulldown followed by LC-MS/MS. Red inset depicts a set of phosphatase subunits and phosphatase regulatory proteins, green inset highlighting components of myosin complexes, and blue inset showing members of ERM proteins. (B) Immunostaining of RPE1 cells expressing PCDH7-GFP (green), MYPT1 (PPP1R12A) (red) and fluorescent phalloidin (blue). (C) Immunoblotting for GFP pulldown of EGFP-PP1CB-MYPT1 fusion, EGFP-PP1CB*-MYPT1 (D63A) mutant and control construct with cotransfection of PCDH7c-V5 construct. Membranes were blotted using anti-V5, anti-MYPT1, anti-GFP and anti-tubulin antibodies. I (Input), FT(Flow Through), E (Elution) (D) Quantification of proximity ligation assay (PLA) showing fluorescence dots per cell for PCDH7- GFP (anti-GFP) and MYPT1 (anti-MYPT1) interaction (n=29) along with single primary antibody controls, MYPT1 (n=30) and GFP (n=30). ****p<0.0001.

Journal: bioRxiv

Article Title: PCDH7 Promotes Cell Migration by Regulating Myosin Activity

doi: 10.1101/2021.09.21.460794

Figure Lengend Snippet: (A) Depiction of relevant clusters of PCDH7 isoform-c interactome determined by GFP mediated affinity pulldown followed by LC-MS/MS. Red inset depicts a set of phosphatase subunits and phosphatase regulatory proteins, green inset highlighting components of myosin complexes, and blue inset showing members of ERM proteins. (B) Immunostaining of RPE1 cells expressing PCDH7-GFP (green), MYPT1 (PPP1R12A) (red) and fluorescent phalloidin (blue). (C) Immunoblotting for GFP pulldown of EGFP-PP1CB-MYPT1 fusion, EGFP-PP1CB*-MYPT1 (D63A) mutant and control construct with cotransfection of PCDH7c-V5 construct. Membranes were blotted using anti-V5, anti-MYPT1, anti-GFP and anti-tubulin antibodies. I (Input), FT(Flow Through), E (Elution) (D) Quantification of proximity ligation assay (PLA) showing fluorescence dots per cell for PCDH7- GFP (anti-GFP) and MYPT1 (anti-MYPT1) interaction (n=29) along with single primary antibody controls, MYPT1 (n=30) and GFP (n=30). ****p<0.0001.

Article Snippet: For PCDH7- V5 constructs, PCDH7b and PCDH7c were cloned into PLEX_307 (Addgene#41392). eGFP- PP1CB-MYPT1 and eGFP-PP1CB(D63A)-MYPT1 were provided by Mathieu Bollen from KU, Leuven and generated as previously described ( ).

Techniques: Liquid Chromatography with Mass Spectroscopy, Immunostaining, Expressing, Western Blot, Mutagenesis, Construct, Cotransfection, Proximity Ligation Assay, Fluorescence